Category

Antibody

Concentration

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Form

Purified antibody

Conjugation

Biotin Conjugate

Clone

Polyclonal antibody

Recognised antigen

HP1 gamma / Heterochromatin protein 1 gamma (Biotin Conjugate)

Host animal

Goat (Capra aegagrus hircus)

Clonality

Polyclonal (goat origin)

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Tested applications

WB, ELISA (peptide)

Recommended dilutions

Western blot: 0.1-0.3 ug/ml,ELISA (peptide) LOD: 1:32000

Notes

Optimal dilution of the HP1 gamma antibody should be determined by the researcher.1. This HP1 gamma antibody does not cross react with HP1-alpha or beta.

Intented use

This HP1 gamma antibodyis to be used only for research purposes and not for diagnostics..

Gene ID #

11335

Purity

Antigen affinity

Description

Additional name(s) for this target protein: Heterochromatin protein 1 gamma; CBX3

Immunogen

Amino acids EAFLNSQKAGKEKD were used as the immunogen for this HP1 Gamma antibody.

Storage

Aliquot and store the HP1 gamma antibody at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Additional conjugation

Biotinylated

French translation

anticorps