Bovine, Chicken, Human (Homo sapiens), Monkey, Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes DNA polymerase e (Pol e) at> 200kD. Also recognizes a 140kD form of Pol e, known as Pol e. Species Reactivity: Detects both Pol e and Pol e of Human, Monkey, bovine and Mouse. A mixture of clones 0.N.250 and 0.N.251 will recognize Pol e from D. melanogaster (Pol e only), T. ni insect cells (Pol e only), S. cerevisiae (Pol e and Pol e), X. laevis (Pol e only) and Chicken (Pol e only).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Affinity Purified by Protein G affinity chromatography.
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Storage and shipping
Store the antibody at -20 degrees Celsius.
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MBS Monoclonals supplies azide free antibodies on request.Recently MBS Monoclonals made this DNA Polymerase, epsilon (Polepsilon, CHRAC 17, Chromatin Accessibility Complex 17, DNA Polymerase Epsilon p17 Subunit, DNA Polymerase Epsilon Subunit 3, 17, YBL1) (BSA ) available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.