Our specialists from Gentaur/Genprice advise to follow carefully the instructions for use and store the EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit in refrigerator and according to the label upon arrival.
Manufactured in United States. Designed for Research Use Only, not for human or animal consumption.
Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The Epigen antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials.
Chromatin isolation for histone extracts like H2A, H2B, H3, and H4 extraction for SDS-PAGE electrophoresis. Histones act as spools around which DNA winds, and also play a role in gene regulation. The core histones include H2A, H2B, H3, and H4. Histones undergo post-translational modifications, which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have long tails protruding from the nucleosome, which can be covalently modified at several places (i.e., methylation, acetylation, phosphorylation, etc.). Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis). Combinations of modifications are thought to constitute a "histone code" and an understanding of this code may help to answer questions about human development and disease.